How to choose the right Collagenase Cell Isolation Enzyme
Posted by Sara On Tue 13 Aug, 2019

How to choose the right Collagenase cell isolation enzyme by Sara Liebowitz



Keep these things in mind when you are choosing a cell isolation enzyme:

 

  1. There are a number of manufacturers and distributors that supply collagenase cell isolation enzymes but the enzymes that each enzyme manufacturer produces for the same cell isolation application are not the same, they do not have the same name, and in most cases they are supplied in different units.

 

  1. Some collagenase cell isolation enzymes include a collagenase and neutral protease combined while some collagenase cell isolation enzymes require you to purchase a collagenase and neutral protease separately. There is also a clostripain enzyme which is sold separately to help you "fine tune" your isolation.

 

  1. Enzymes are also produced in batches so each batch may not isolate as many viable cells as the last batch. The batches are categorized as lots. You can obtain a Certificate of Analysis (CoA) for each lot to determine the activity level of the enzyme.

 

  1. Most manufacturers and distributors list on their website the type of Collagenase they supply based on your application. Keep in mind whether your application is research or clinical based. All enzyme manufacturers produce both research and clinical grade enzymes for each application and they will provide you with a protocol.

Most researchers and clinicians try a research grade enzyme first and then move up to a clinical grade enzyme because of the cost differential.

Clinical grade enzymes are available in animal free, animal free cGMP or cGMP grades. Animal free clinical enzymes are produced to eliminate the potential of animal derived pathogens. GMP (Good Manufacturing Practices) enzymes are produced in compliance with Good Manufacturing Practices guidelines.

  1. Lastly, look for an enzyme that is easy to use, stable and consistent. 
Crescent Chemical Co., Inc. supplies Nordmark Collagenases, Neutral Proteases and Clostripain. Click our link below to learn more about our collagenase cell isolation enzymes.

http://www.creschem.com/collagenase--neutral-protease-and-clostripain-for-tissue-dissociation-and-cell-isolation-h-b-content.aspx

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