We do not recommend a specific buffer since we know that several buffer types, including PBS, Tris, and HBSS work for cell isolation with collagenase.

Collagenase depends on calcium, therefore it is recommended to use a buffer with ≥ 2 mM Ca2+. Collagenase is inhibited by EDTA thus absolutely no calcium chelating agents (e.g. EDTA) should be contained in the buffer.