Crescent Calculator for SERVA Collagenase NB

SERVA Collagenase NB is designed for dissociation of different tissues to isolate various cell types. For isolation of each cell type, a specific isolation procedure has to be applied to receive optimal yields of viable cells. This SERVA Collagenase NB Calculator developed by Crescent Chemical Co., Inc. will help you to achieve the recommended enzymatic activity in your buffer. SERVA offers high quality collagenase with reliable lot-to-lot consistency. The activity in PZ U/mg of each lot is stated on the Certificate of Analysis which comes with the SERVA Collagenase NB.

  Please enter the collagenase activity of your current lot:
    PZ U/mg.

To establish the cell isolation procedure, SERVA has recommended a starting concentration and digestion time corresponding to the particular cell type.

  Please enter the collagenase concentration you would like to start with:
    PZ U/ml.

  How much collagenase solution do you want to prepare?