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Hydranal® Discover The Perfect Formula


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HYDRANAL®
BROCHURE


HYDRANAL® BROCHURE

HYDRANAL®
Manual


HYDRANAL® BROCHURE

CRESCENT CHEMICAL COMPANY

 

 

 

 

Collagenase NB, Neutral Protease NB, Collagenase N Reference Products and Anti-Collagenase Antibodies properties and applications include:


NB 1 Premium Grade
Cat. No. 17455.03
Pack Size ≥ 2,000 PZ U/Vial
  • Chromatographically highly purified
  • Class l and ll collagenases
  • Use in combination with NB Neutral Protease
  • PZ activity * ≥ 3.0 U/mg lyophilisate
  • Largely free of clostripain, trypsin-like proteases
  • Endotoxins ≥ 10 EU/mg lyophilisate
  • Collagen characterization
  • Isolation of pancreatic islets
  • Use in combination with Neutral Protease NB
  • Collagen characterization

NB 1 GMP Grade
Cat. No. 17542.01
Pack Size ≥ 2,000 PZ U/Vial
  • Enzymatic properties see NB 1 Premium Grade
  • Manufactured according to Intl. cGMP guidelines
  • Isolation of pancreatic islets for transplantation into humans
  • Use in combination with Neutral Protease NB GMP Grade

AF-1 GMP Grade
Cat. No. 17457-01
Pack Size ≥ 2,000 PZ U/Vial
  • Chromatographically highly purified
  • Class l and ll collagenases
  • Very low levels of endotoxins
  • Isolation of pancreatic islets sensitive liver, dental pulp cells

NB 4 Standard Grade
Cat. No. 17454.01/ 1 g
Cat. No. 17454.02/ 500 mg
Cat. No. 17454.03/ 5 g
  • Class l and ll collagenases and a balanced ratio of proteolytic activities
  • PZ activity* ≥ 0.1 U/mg lyophilisate
  • Isolation of various cell types including neuronal and endothelial cells, chondrocytes, adipocytes epithelial cells, stem cells, gangliocytes and periodontal cells

NB 4G Proved Grade
Cat. No. 17465.01/ 500 mg
Cat. No. 17465.02// 1 g
Cat. No. 17465.03/ 5 g
  • Class l and ll collagenases as well as a balanced mix of neutral protease, clostripain, and tryptic activities
  • PZ act.* ≥ 0.18 U/mg lyophilisate
  • Fuction tested for cell isolation
  • Isolation of adipocytes, fibroblasts hepatocytes, chondrocytes, stem cells, frog oocytes, mastocytes and tumor cells

NB 5 Sterile Grade
Cat. No. 17459.03 / 1 g
  • Enzymatic properties see NB 4
  • Sterile according to European req. For medicinal prods. (Pharm. Eur.)
  • Application fields see NB 4
  • Sterile and ready to use

NB 6 GMP Grade
Cat. No. 17458.03/ 1 g
Cat. No. 17458.04/ 100 mg
  • Enzymatic properties see NB 4
  • Aseptic production according to
  • European req. for medicinal prods.(Pharm. Eur.)
  • Manufactured according to Intl. cGMP guidelines
  • Application fields see NB 4
  • Production of cell as well as tissue engineering for transplantation into humans

NB 8 Broad Range
Cat. No. 17456.01/ 250 mg
Cat. No. 17456.02/ 1 g
  • Class l and ll collagenases and reduced levels of clostripain, trypsin-like activities and neutral protease
  • PZ act.* ≥ 0.9 U/mg lyophilisate
  • Endotoxins ≥ 100 EU/mg lyophilisate
  • Isolation of cardiomyocytes, porcine hepatocytes, rodent pancreatic islets tumor and thyroid cells

NB Neutral Protease
Cat. No. 30301.11/ 50 U (DMC)
Cat. No. 30301.12/ 100 U (DMC)
  • Chromatographically purified neutral protease.
  • Activity (DMC assay) ≥ 0.50 U/mg
  • Tissue dissociation
  • Cell isolation in combination with Collagenase NB

NB Neutral Protease
High Active Grade

Cat. No. 30302.02/ 80 U (DMC)
  • Chromatographically purified and contains an increased proteolytic activity
  • Largely free from collagenolytic activity (PZ U)
  • Endotoxin level is very low
  • Activity (DMC assay) ≥ 4.00 U/mg
  • Endotoxin ≥ 50.0 EU/mg
  • Tissue dissociation
  • Cell isolation in combination with Collagenase NB 1 Premium Grade or another Collagenase NB product

NB Neutral Protease GMP Grade
Cat. No. 17452.01/ ≥ 2,000 U (PZ)
  • Chromatographically purified and largely free from collagenolytic activity (PZ U) and endotoxins
  • Activity (DMC assay) ≥ 0.50 U/mg
  • Tissue dissociation
  • Cell isolation in combination with Collagenase NB 1 GMP Grade or Collagenase NB 6 GMP Grade from various tissue types for clinical applications.

NB Neutral Protease AF GMP Grade
Cat No. 30306.01/ ≥ 2,000 U (PZ)
  • Chromatographically purified and largely free from collagenolytic activity (PZ U) as well as endotoxins
  • Absent of any animal-derived ingredients and thorough microbial analysis provide the highest possible safety
  • Activity (DMC assay) ≥ 0.50 U/mg
  • Tissue dissociation
  • Cell isolation in combination with Collagenase AF-1 GMP Grade from several tissue types for clinical applications

Collagenase N
Reference Content / ≥ 50 mg
Cat. No. 17463
  • Class l and ll collagenases and other proteolytic enzymes
  • Collagenase activity is regularly reevaluated
  • Reference substance for enzyme activity determination.

Collagenase N
Reference Identity / ≥ 50 mg
Cat. No. 17464
  • Class I and ll collagenases and other proteolytic enzymes
  • Reference substance for SDS-PAGE analysis, for incoming goods inspection according to cGMP

Anti-Collagenase
Cat. No. 58050 / 100 μ
  • Specifically against collagenases from C. histolyticum
  • lgG fraction purified by Protein G affinity chromatography
  • Use in ELISA and Western Blot experiments
  • Immunostaining of tissue
  • Analysis of residual collagenase impurities in cell preparations

Anti-Collagenase HRP
Cat. No. 58051 / 100 μ
  • Specifically against collagenases from C. histolyticum
  • lgG fraction purified by affinity chromatography
  • Conjugated with horseradish
  • Peroxidase
  • Use in ELISA and Western Blot experiments
  • Immunostaining of tissue
  • Analysis of residual collagenase impurities in cell preparations


* PZ activity acc. to Wϋnsch, measured at 25 ° C.


Crescent Chemical Co., Inc. is the distributor of Nordmark Collagenase NB and Neutral Protease NB in the US and Canada. Nordmark Collagenase NB are particularly well suited for various tissue dissociation and cell isolation applications. Nordmark is the world's largest collagenase producer. Products are manufactured under strict pharmaceutical regulations which guarantees stringent quality control, reliable lot-to-lot consistency and excellent product performance. TSE safety of the fermentation product has been certified by the European Authorities, EDQM and the supplier’s certificate of origins.

Drug Master File for Collagenase

Click for technical information

Crescent Chemical Co., Inc. is the distributor of Nordmark Collagenase NB, Neutral Protease NB and Clostripain NB in Americas. Nordmark is the world's largest Collagenase producer. Nordmark Collagenase NB and Neutral Protease NB products are particularly well suited for various tissue dissociation and cell isolation applications. Nordmark Collagenase NB and Neutral Protease NB products are manufactured under strict pharmaceutical regulations which guarantees stringent quality control, reliable lot-to-lot consistency and excellent product performance. TSE safety of the fermentation product has been certified by the European Authorities, EDQM and the supplier’s certificate of origins.
Crescent Chemical Co., Inc. is Nordmark's Clostripain distributor in the Americas. Clostripain NB is, in combination with Collagenase NB 1 Premium Grade and Neutral Protease NB, especially suited for isolation of islets of Langerhans from human pancreas. Clostripain NB can also be used in combination with Collagenase NB 4 Standard Grade and NB 8 Broad Range Grade for cell isolation from several other human or animal tissues. Fine tune your isolation with Clostripain NB.


Crescent Chemical Co., Inc. is the US distributor for SERVA Electrophoresis GmbH, a leading manufacturer of specialty reagents for SDS, Native PAGE electrophoresis and Isoelectric Focusing. SERVA is also a certified ISO 9001 supplier of biochemical products. All Serva products are subject to stringent quality control to meet the specifications stated in the Certificate of Analysis provided with each product.

Electrophoresis by SERVA Serva 2018 Catalog Market Square
SERVA ELECTROPHORESIS Serva 2018 Catalog Market Square

Click The Links Below For More Information.



Serva Products.

"Facts About 2D Gel Electrophoresis"

BlueLine Equipment

SDS PreNets Blotting Kit

SERVA Proteome Markers, 5 Vials

SERVA 2D Gel Electrophoresis in Mini Format

BlueHorizon Super Cool Flatbed System

BlueFlash Semi-Dry Blotter

GEL-FIX / NetFix

SERVA ICPL Quadruplex Kit

SERVA IPG BlueStrips

Protease Inhibitor Mixes For Protein Research

SerVaGel

SERVALYT Carrier Ampholytes

Trypsin NB Premium Grade, MS approved

Certificate ISO 9001:2008 (english)












Serva FAQ

Ultroser G™ Serum Substitute #67042

Ultroser G Serum Substitute Crescent Chemical Co., Inc. can supply Ultroser G Serum Substitute. Ultroser G is manufactured in France. Importation of Ultroser G requires a USDA Importation Permit. Crescent Chemical is happy to help you apply for this permit.

Description

Ultroser™ G serum substitute can successfully replace FCS (fetal calf serum) in cell culture medium. It is designed for the in vitro culture of anchorage-dependent cells. It is specially tested as the primary culture from amniotic liquid cells and is designed to confirm their ability for karyotyping.

Ultroser G serum substitute is CE mark-certified.

Features and Benefits
  • Semi-defined composition ensuring batch-to-batch reproducibility
  • Low protein content
  • Easy use and storage

Ultroser G serum substitute has a concentration five times higher than fetal calf serum (2 % of reconstituted Ultroser G serum substitute is equivalent to 10% fetal calf serum in the basal medium).

Quality Control Tests
Tests usually performed on Ultroser G serum substitute are:

  • Physicochemical parameters
  • pH
  • Protein concentration
  • Sterility control
  • Bacteria & Fungi
  • Bovine viruses (BHV1-DVB/MM, hemadsorbents)
  • Mycoplasma
  • Proliferation capacity on Hep G2
  • Karyotyping

Applications

Cells successfully tested with Ultroser™ G serum substitute:

  • Glial cells
  • rat and human myocytes
  • Amniotic cells
  • Vero
  • CHO
  • BHK
  • Bone marrow cells
  • Fibroblasts
  • skin cells
  • HeLa
  • MRC-5
  • Thyroïd cells
  • Chondrocytes
  • Arterial cells
  • Leydig cells
  • Lewis cells
  • Promyelocytes HL60
  • Raji
  • P3HR1
  • Astrocytes
  • Erythroblasts
  • Hypothalamic cells
  • Keratinocytes
It is particularly useful in prenatal diagnosis for the culture of amniotic cells for karyotyping.

Ultroser G serum substitute is available as a lyophilized powder. The product is shipped at ambient temperature and must be stored at 2-8 degrees C.