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Collagenases obtained from Clostridium histolyticum are enzymes that break the peptide bond in collagen. Clostridium histolyticum secretes Collagenase Class I and II, Neutral Protease and Clostripain.
These four enzymes work together to dissociate the extracellular matrix for cell isolation from tissue for clinical and research applications.
Crescent Chemical Co., Inc. supplies NORDMARK Animal-free GMP, GMP, and NB Collagenase, Neutral Protease and Clostripain for cell isolation and passaging. Nordmark is the largest producer of collagenase worldwide.
Click Here For More Information on Collagenase
Click here for Collagenase Calculator

Collagenase AF-1 GMP Grade (The World's First)
The AF-1 GMP clinical grade Collagenase is completely animal free and is produced to eliminate the potential of animal derived pathogens.
Click here for Collagenase AF-1 GMP Grade Product Notes

Neutral Protease AF GMP Grade (The World's First)
The AF GMP clinical grade Neutral Protease is completely animal free and is produced to eliminate the potential of animal derived pathogens.
Click here for Neutral Protease AF GMP Grade Product Notes

Collagenase NB 1 GMP Grade
Recommended for clinical applications which have to meet GMP requirements like tissue engineering and transplantation into humans.
Click here for Collagenase NB 1 GMP Product Notes

Neutral Protease NB GMP Grade
Recommended for clinical applications which have to meet GMP requirements like tissue engineering and transplantation into humans.
Click here for Neutral Protease NB GMP Product Notes

Collagenase NB 1 Premium Grade
This highly purified collagenase grade in combination with Neutral Protease NB is used for isolation of pancreatic islets of Langerhans from different species.
Click here for Collagenase NB 1 Premium Grade Product Notes

Neutral Protease NB
Neutral Protease NB is used in combination with purified Collagenase NB 1 Premium Grade, Collagenase NB 4 Standard Grade or Collagenase NB 8 Broad Range.
Click here for Neutral Protease Product Notes

Neutral Protease NB High Active Grade
Neutral Protease NB High Active Grade contains an increased proteolytic activity and is used in combination with highly purified Collagenase NB 1 Premium Grade, Collagenase NB 4 Standard Grade or Collagenase NB 4G Proved Grade.
Click here for Neutral Protease High Active Grade Product Notes

Collagenase NB 4 Standard Grade
Applicable to research tissue dissociation such as isolation of human ADSCs, stem cells, chondrocytes, fibroblasts, neuronal, endothelial hepatocytes, cardiomyocytes, tumor, muscle, epithelial, gangliocytes, oocytes and periodontal cells.
Click here for Collagenase NB 4 Standard Grade Product Notes

Collagenase NB 4G Proved Grade
Suitable for isolation of a broad variety of cell types including adipocytes, fibroblasts, hepatocytes, chondrocytes, oocytes and stem cells.
Click here for Collagenase NB 4G Proved Grade Product Notes

Collagenase NB 5 Sterile Grade
Applicable to tissue dissociation such as isolation of human and rodent hepatocytes, chondrocytes, neuronal and endothelial cells, fibroblasts, cells from adipose tissue (e.g. ADSCs), stem cells and rodent osteoblasts.
Click here for Collagenase NB 5 Sterile Grade Product Notes

Collagenase NB 6 GMP Grade
Applicable to clinical tissue dissociation such as isolation of human ADSCs, stem cells, chondrocytes, fibroblasts, neuronal, endothelial hepatocytes, cardiomyocytes, tumor, muscle, epithelial, gangliocytes, oocytes and periodontal cells.
Click here for Collagenase NB 6 GMP Product Notes

Collagenase NB 8 Broad Range
Increased collagenase activity. Applicable to tissue dissociation such as isolation of cardiomyocytes, porcine hepatocytes, rodent pancreatic tumors and thyroid cells.
Click here for Collagenase NB 8 Broad Range Grade Product Notes

Clostripain NB
Used in combination with other Collagenases and Neutral Proteases to improve your isolation results from human and animal tissue.
Click here for Clostripain NB Product Notes

Collagenase Properties and Applications, Collagenase Calculator, Drug Master File (DMF) and Frequently asked questions (FAQ):


Collagenase NB 1 Premium Grade
Cat. No. 17455.03
Pack Size ≥ 2,000 PZ U/Vial
  • Chromatographically highly purified
  • Class l and ll collagenases
  • Use in combination with NB Neutral Protease
  • PZ activity * ≥ 3.0 U/mg lyophilisate
  • Largely free of clostripain, trypsin-like proteases
  • Endotoxins ≥ 10 EU/mg lyophilisate
  • Collagen characterization
  • Isolation of pancreatic islets
  • Use in combination with Neutral Protease NB
  • Collagen characterization

Collagenase NB 1 GMP Grade
Cat. No. 17542.01
Pack Size ≥ 2,000 PZ U/Vial
  • Enzymatic properties see NB 1 Premium Grade
  • Manufactured according to Intl. cGMP guidelines
  • Isolation of pancreatic islets for transplantation into humans
  • Use in combination with Neutral Protease NB GMP Grade

Collagenase AF-1 GMP Grade
Cat. No. 17457-01
Pack Size ≥ 2,000 PZ U/Vial
  • Chromatographically highly purified
  • Class l and ll collagenases
  • Very low levels of endotoxins
  • Isolation of pancreatic islets sensitive liver, dental pulp cells

Collagenase NB 4 Standard Grade
Cat. No. 17454.01/ 1 g
Cat. No. 17454.02/ 500 mg
Cat. No. 17454.03/ 5 g
  • Class l and ll collagenases and a balanced ratio of proteolytic activities
  • PZ activity* ≥ 0.1 U/mg lyophilisate
  • Isolation of various cell types including neuronal and endothelial cells, chondrocytes, adipocytes epithelial cells, stem cells, gangliocytes and periodontal cells

Collagenase NB 4G Proved Grade
Cat. No. 17465.01/ 500 mg
Cat. No. 17465.02// 1 g
Cat. No. 17465.03/ 5 g
  • Class l and ll collagenases as well as a balanced mix of neutral protease, clostripain, and tryptic activities
  • PZ act.* ≥ 0.18 U/mg lyophilisate
  • Fuction tested for cell isolation
  • Isolation of adipocytes, fibroblasts hepatocytes, chondrocytes, stem cells, frog oocytes, mastocytes and tumor cells

Collagenase NB 5 Sterile Grade
Cat. No. 17459.03 / 1 g
  • Enzymatic properties see NB 4
  • Sterile according to European req. For medicinal prods. (Pharm. Eur.)
  • Application fields see NB 4
  • Sterile and ready to use

Collagenase NB 6 GMP Grade
Cat. No. 17458.03/ 1 g
Cat. No. 17458.04/ 100 mg
  • Enzymatic properties see NB 4
  • Aseptic production according to
  • European req. for medicinal prods.(Pharm. Eur.)
  • Manufactured according to Intl. cGMP guidelines
  • Application fields see NB 4
  • Production of cell as well as tissue engineering for transplantation into humans

Collagenase NB 8 Broad Range
Cat. No. 17456.01/ 250 mg
Cat. No. 17456.02/ 1 g
  • Class l and ll collagenases and reduced levels of clostripain, trypsin-like activities and neutral protease
  • PZ act.* ≥ 0.9 U/mg lyophilisate
  • Endotoxins ≥ 100 EU/mg lyophilisate
  • Isolation of cardiomyocytes, porcine hepatocytes, rodent pancreatic islets tumor and thyroid cells

Neutral Protease NB
Cat. No. 30301.11/ 50 U (DMC)
Cat. No. 30301.12/ 100 U (DMC)
  • Chromatographically purified neutral protease.
  • Activity (DMC assay) ≥ 0.50 U/mg
  • Tissue dissociation
  • Cell isolation in combination with Collagenase NB

Neutral Protease NB High Active Grade

Cat. No. 30302.02/ 80 U (DMC)
  • Chromatographically purified and contains an increased proteolytic activity
  • Largely free from collagenolytic activity (PZ U)
  • Endotoxin level is very low
  • Activity (DMC assay) ≥ 4.00 U/mg
  • Endotoxin ≥ 50.0 EU/mg
  • Tissue dissociation
  • Cell isolation in combination with Collagenase NB 1 Premium Grade or another Collagenase NB product

Neutral Protease GMP Grade
Cat. No. 17452.01/ ≥ 2,000 U (PZ)
  • Chromatographically purified and largely free from collagenolytic activity (PZ U) and endotoxins
  • Activity (DMC assay) ≥ 0.50 U/mg
  • Tissue dissociation
  • Cell isolation in combination with Collagenase NB 1 GMP Grade or Collagenase NB 6 GMP Grade from various tissue types for clinical applications.

Neutral Protease AF GMP Grade
Cat No. 30306.01/ ≥ 2,000 U (PZ)
  • Chromatographically purified and largely free from collagenolytic activity (PZ U) as well as endotoxins
  • Absent of any animal-derived ingredients and thorough microbial analysis provide the highest possible safety
  • Activity (DMC assay) ≥ 0.50 U/mg
  • Tissue dissociation
  • Cell isolation in combination with Collagenase AF-1 GMP Grade from several tissue types for clinical applications

Collagenase N
Reference Content / ≥ 50 mg
Cat. No. 17463
  • Class l and ll collagenases and other proteolytic enzymes
  • Collagenase activity is regularly reevaluated
  • Reference substance for enzyme activity determination.

Collagenase N
Reference Identity / ≥ 50 mg
Cat. No. 17464
  • Class I and ll collagenases and other proteolytic enzymes
  • Reference substance for SDS-PAGE analysis, for incoming goods inspection according to cGMP

Anti-Collagenase
Cat. No. 58050 / 100 μ
  • Specifically against collagenases from C. histolyticum
  • lgG fraction purified by Protein G affinity chromatography
  • Use in ELISA and Western Blot experiments
  • Immunostaining of tissue
  • Analysis of residual collagenase impurities in cell preparations

Anti-Collagenase HRP
Cat. No. 58051 / 100 μ
  • Specifically against collagenases from C. histolyticum
  • lgG fraction purified by affinity chromatography
  • Conjugated with horseradish
  • Peroxidase
  • Use in ELISA and Western Blot experiments
  • Immunostaining of tissue
  • Analysis of residual collagenase impurities in cell preparations


* PZ activity acc. to Wϋnsch, measured at 25 ° C.
Drug Master File for Collagenase





Serva FAQ
Neutral Protease from Clostridium histolyticum is a proteolytic enzyme that can cleave peptide bonds in proteins. Due to these properties, it is suitable for the isolation of a broad range of cell types by tissue dissociation.

Crescent Chemical Co., Inc. supplies NORDMARK Animal-free GMP, GMP and NB Neutral Protease.

Neutral Protease AF GMP Grade (The World's First)
The AF GMP clinical grade Neutral Protease is completely animal free and is produced to eliminate the potential of animal derived pathogens.
Click here for Neutral Protease AF GMP Product Notes

Neutral Protease NB GMP Grade
Recommended for clinical applications which have to meet GMP requirements like tissue engineering and transplantation into humans.
Click here for Neutral Protease NB GMP Product Notes

Neutral Protease NB
Neutral Protease NB is used in combination with purified Collagenase NB 1 Premium Grade, Collagenase NB 4 Standard Grade or Collagenase NB 8 Broad Range.
Click here for Neutral Protease Product Notes

Neutral Protease NB High Active Grade
Neutral Protease NB High Active Grade has a higher activity and higher purity compared to Neutral Protease NB. It is used in combination with highly purified Collagenase NB 1 Premium Grade, Collagenase NB 4 Standard Grade or Collagenase NB 4G Proved Grade.
Click here for Neutral Protease High Active Grade Product Notes

Clostripain also known as Clostridiopeptidase B or Endoproteinase Arg-C is a proteinase that cleaves peptide bonds on the carboxyl side of arginine. This protein is a heterodimer composed of a heavy and a light chain that are held together via strong noncovalent forces. As a proteolytic enzyme it can be used for isolation of various cell types by tissue dissociation.

Clostripain exists in two different forms, an oxidized, native condition and a reduced activated form that differ in enzymatic activity. The native clostripain can be activated with reducing agents (e.g. DTT, Cysteine) resulting in a higher activity of the enzyme.

Crescent Chemical Co., Inc. supplies NORDMARK CLOSTRIPAIN NB.
Click here for Clostripain NB Product Notes

Clostripain, the Missing Link in the Enzyme Blend for Efficient Human Islet Isolation
2017 - IPITA Islet Isolation
Synergistic Effect of Neutral Protease and Clostripain on Rat Pancreatic Islet Isolation



Crescent Chemical Co., Inc. is the US distributor for SERVA Electrophoresis GmbH, a leading manufacturer of specialty reagents for SDS, Native PAGE electrophoresis and Isoelectric Focusing. SERVA is also a certified ISO 9001 supplier of biochemical products. All Serva products are subject to stringent quality control to meet the specifications stated in the Certificate of Analysis provided with each product.

Electrophoresis by SERVA Serva 2018 Catalog Market Square
SERVA ELECTROPHORESIS Serva 2018 Catalog Market Square
2D Electrophoresis Protein Sample Preparation SERVAGel
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Serva Products.

"Facts About 2D Gel Electrophoresis"

Blue Horizon

SDS PreNets Blotting Kit

SERVA Proteome Markers, 5 Vials

SERVA 2D Gel Electrophoresis in Mini Format

BlueHorizon Super Cool Flatbed System

BlueFlash Semi-Dry Blotter

GEL-FIX / NetFix

SERVA ICPL Quadruplex Kit

SERVA IPG BlueStrips

Protease Inhibitor Mixes For Protein Research

SerVaGel

SERVALYT Carrier Ampholytes

Trypsin NB Premium Grade, MS approved

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Ultroser G™ Serum Substitute #67042

Ultroser G Serum Substitute Crescent Chemical Co., Inc. can supply Ultroser G Serum Substitute. Ultroser G is manufactured in France. Importation of Ultroser G requires a USDA Importation Permit. Crescent Chemical is happy to help you apply for this permit.

Description

Ultroser™ G serum substitute can successfully replace FCS (fetal calf serum) in cell culture medium. It is designed for the in vitro culture of anchorage-dependent cells. It is specially tested as the primary culture from amniotic liquid cells and is designed to confirm their ability for karyotyping.

Ultroser G serum substitute is CE mark-certified.

Features and Benefits
  • Semi-defined composition ensuring batch-to-batch reproducibility
  • Low protein content
  • Easy use and storage

Ultroser G serum substitute has a concentration five times higher than fetal calf serum (2 % of reconstituted Ultroser G serum substitute is equivalent to 10% fetal calf serum in the basal medium).

Quality Control Tests
Tests usually performed on Ultroser G serum substitute are:

  • Physicochemical parameters
  • pH
  • Protein concentration
  • Sterility control
  • Bacteria & Fungi
  • Bovine viruses (BHV1-DVB/MM, hemadsorbents)
  • Mycoplasma
  • Proliferation capacity on Hep G2
  • Karyotyping

Applications

Cells successfully tested with Ultroser™ G serum substitute:

  • Glial cells
  • rat and human myocytes
  • Amniotic cells
  • Vero
  • CHO
  • BHK
  • Bone marrow cells
  • Fibroblasts
  • skin cells
  • HeLa
  • MRC-5
  • Thyroïd cells
  • Chondrocytes
  • Arterial cells
  • Leydig cells
  • Lewis cells
  • Promyelocytes HL60
  • Raji
  • P3HR1
  • Astrocytes
  • Erythroblasts
  • Hypothalamic cells
  • Keratinocytes
It is particularly useful in prenatal diagnosis for the culture of amniotic cells for karyotyping.

Ultroser G serum substitute is available as a lyophilized powder. The product is shipped at ambient temperature and must be stored at 2-8 degrees C.